Identification, localization, and functional analysis of the homologues of mouse CABS1 protein in porcine testis

DOI 機関リポジトリ HANDLE Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献2件 参考文献28件 オープンアクセス
  • Shawki Hossam H.
    Laboratory of Reproductive Biochemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan Department of Animal Genetic Resources, National Gene Bank, Egypt
  • Kigoshi Takumi
    Laboratory of Reproductive Biochemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan
  • Katoh Yuki
    Center for Humanities and Sciences, Ibaraki Prefectural University of Health Sciences, Japan
  • Matsuda Manabu
    Laboratory of Reproductive Biochemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan
  • Ugboma Chioma M.
    Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  • Takahashi Satoru
    Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  • Oishi Hisashi
    Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  • Kawashima Akihiro
    Laboratory of Reproductive Biochemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan

この論文をさがす

抄録

Previously, we have identified a calcium-binding protein that is specifically expressed in spermatids and localized to the flagella of the mature sperm in mouse, so-called mCABS1. However, the physiological roles of CABS1 in the male reproductive system have not been fully elucidated yet. In the current study, we aimed to localize and clarify the role of CABS1 in porcine (pCABS1). We determined for the first time the full nucleotides sequence of pCABS1 mRNA. pCABS1 protein was detected on SDS-PAGE gel as two bands at 75 kDa and 70 kDa in adult porcine testis, whereas one band at 70 kDa in epididymal sperm. pCABS1 immunoreactivity in seminiferous tubules was detected in the elongated spermatids, and that in the epididymal sperm was found in the acrosome as well as flagellum. The immunoreactivity of pCABS1 in the acrosomai region disappeared during acrosome reaction. We also identified that pCABS1 has a transmembrane domain using computational prediction of the amino acids sequence. The treatment of porcine capacitated sperm with anti-pCABS1 antiserum significantly decreased acrosome reactions. These results suggest that pCABS1 plays an important role in controlling calcium ion signaling during the acrosome reaction.

収録刊行物

  • Experimental Animals

    Experimental Animals 65 (3), 253-265, 2016

    公益社団法人 日本実験動物学会

被引用文献 (2)*注記

もっと見る

参考文献 (28)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ