<b>Molecular Interaction of the Analogous Peptide SspB (390-T400K-402) derived from </b><b><i>Streptococcus gordonii </i></b><b>Surface Protein Peptide with Periodontal Bacteria </b>

DOI Web Site 参考文献36件
  • Ichinosawa Takahiro
    Nihon University Graduate School of Dentistry at Matsudo, Pediatric Dentistry, Matsudo, Chiba 271-8587, Japan
  • Ito Tatsuro
    Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Yonezawa Hideo
    Department of Infectious Disease, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
  • Senpuku Hidenobu
    Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan
  • Shimizu Takehiko
    Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

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<p>The initial stages of dental biofilm formation involved the adherence of early colonizing organisms such as Streptococcus gordonii on the saliva-coated tooth surface. The surface protein SspB of S. gordonii is known to play a role in adherence to salivary protein and mediates coaggregation with other bacteria. We previously demonstrated that the analogous SspB peptide, SspB(390-T400K-402), effectively bound to salivary agglutinin peptide SRCRP2 (scavenger receptor cysteine-rich domain peptide 2). To investigate molecular interaction of supra- and sub-gingival dental plaque biofilm formation among streptococci, periodontal bacteria, and salivary agglutinin as a unit, we examined the binding activity of the SspB(390-T400K-402)peptide to periodontal bacteria. The binding activity of SspB(390-T400K-402)was detected by ELISA. The SspB(390-T400K-402) peptide bound to P. gingivalis ATCC 33277, F. nucleatum ATCC 23726, and F. nucleatum #20. This binding was reduced by the presence of whole saliva; however, the presence of SRCRP2 enhanced the binding of the SspB peptide to these bacteria. These findings suggest that the binding ability of S. gordonii to these periodontal bacteria may provide,either directly(by attachment to P. gingivalis Mfa1)or indirectly(by attachment to F. nucleatum), a support system for the colonization and the biofilm formation of periodontal bacteria.</p>

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  • ＩＪＯＭＳ

    ＩＪＯＭＳ 15 (3-4), 160-167, 2017

    日本大学松戸歯学部 口腔科学研究所

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