Shoot-Tip Culture of Acerola (<i>Malpighia glabra</i> L.)

• YAMAMOTO Masashi

  Faculty of Agriculture, Kagoshima University

• ITO Akihiro

  Faculty of Agriculture, Kagoshima University

• SOGABE Aki

  Faculty of Agriculture, Kagoshima University

• KAJIWARA Yuki

  Faculty of Agriculture, Kagoshima University

• ISHIHATA Kiyotake

  Faculty of Agriculture, Kagoshima University

抄録

Culture conditions for shoot tips excised from cutting propagated plants of acerola (Malpighia glabra L.) were investigated to develop a shoot-tip culture system. Shoot tips of acerola “Sanmikei (Hosoba)” were grown on a medium, consisting of MS basal components with 3% sucrose. The addition of 0.1 ppm 6-benzylaminopurin (BA), 0.05 ppm BA + 0.05 ppm zeatin or 0.1 ppm BA + 0.1 ppm zeatin was effective for the proliferation of shoots. Those that had grown two to three cm from the subculture, were examined further for rooting, which had been stimulated when the shoots were immersed in 250 ppm indole-3-butyric acid (IBA) for 15 seconds. Ten to eleven days of dark treatment enhanced this. All plantlets were recovered after acclimatization. Sequence-related amplified polymorphism (SRAP) was conducted to validate the genetic homogeneity of the regenerated plants. In SRAP analysis, using 13 primer combinations, no polymorphisms among the regenerated and original plants were detected.

収録刊行物

• Tropical Agriculture and Development

  Tropical Agriculture and Development 61 (2), 57-61, 2017

  日本熱帯農業学会

キーワード

• Barbados cherry
• DNA analysis
• Puerto Rico cherry
• SRAP
• Tissue culture
• West Indian cherry

詳細情報

• CRID: 1390001205298633472
• NII論文ID: 130006900286
• DOI: 10.11248/jsta.61.57
• ISSN: 18828469
• ISSN: 18828450
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可