<Original Article>Hypoxia enhances proliferation through increase of colony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells

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Author(s)

    • 大原 敏之 Ohara Toshiyuki
    • Department of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
    • 宗田 大 Muneta Takeshi
    • Department of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
    • 中川 裕介 Nakagawa Yusuke
    • Department of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
    • 松倉 遊 Matsukura Yu
    • Department of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
    • 古賀 英之 Koga Hideyuki
    • Department of Joint Surgery and Sports Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
    • 辻 邦和 Tsuji Kunikazu
    • Department of Cartilage Regeneration, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

Abstract

Synovial mesenchymal stem cells (MSCs) are anattractive cell source for cartilage and meniscusregeneration. Use of primary MSCs is thepreferable because these cells are safer than cellspassaged several times in terms of probability ofchromosome abnormalities. The effect of hypoxiaon the proliferation of MSCs is controversialand remains unknown in primary synovial MSCs.Primary synovial MSCs were cultured at normoxiaor hypoxia, and colony number, cell number,surface epitopes, mitochondria activity, TEMfinding, and chondrogenic potential were analyzed.To investigate the effect of hypoxia on attachmentof synovial MSCs, cells were cultured at hypoxiafor the first 3 days, then cultured at normoxia. Toinvestigate the effect of hypoxia on proliferation,cells were also cultured at hypoxia for the last11 days. Hypoxia increased colony number andcell number per dish in primary synovial MSCs.Hypoxia did not affect cell number per colony,surface epitopes, mitochondria activity, TEMfinding or chondrogenic potential. Hypoxia for thefirst 3 days did not alter colony number per dishor cell number per dish, while hypoxia for the last11 days increased. Hypoxia enhanced proliferationthrough increase of colony formation rate withchondrogenic potential in primary synovial MSCs.

Journal

  • Journal of Medical and Dental Sciences

    Journal of Medical and Dental Sciences 63(4), 61-70, 2016

    Tokyo Medical and Dental University (TMDU)

Codes

  • NII Article ID (NAID)
    130006944182
  • Text Lang
    ENG
  • ISSN
    1342-8810
  • Data Source
    J-STAGE 
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