Cloning And Characterization Of MADS Box Genes Isolated From <I>Cryptomeria japonica</I> Male Strobili.

• Kurita Manabu

  Forest Tree Breeding Center

• Ohmiya Yasunori

  Forest Tree Breeding Center

• Watanabe Atsushi

  Forest Tree Breeding Center

• Taniguchi Toru

  Kyusyu Regional Breeding Office

• Tsubomura Miyoko

  Forest Tree Breeding Center

• Kondo Teiji

  Forest Tree Breeding Center

Bibliographic Information

Other Title

• スギの花芽で発現するMADS-box遺伝子の機能解析

Abstract

Cryptomeria japonica is one of major conifer species. We consider genetic modification (GM) also can be a powerful tool for breeding. But the potential of GM plants to transfer foreign genes through pollen to related plant species has been cited as an environmental concern. For the purpose of creating male sterile GM Cryptomeria japonica, we are trying to identify genes related to male flower formation. <br>Plants contain a variety of MADS-box genes that play important roles in both the formation of flower meristem and the determination of floral organ identity. We have isolated and characterized 6 MADS-box genes from Cryptomeria japonica using PCR amplification and RACE method. RT-PCR analysis revealed that one of these genes, M8, was expressed stronger in male strobilus and its transcriptional activity was increased according as the development of male strobilus. Now we are trying to determine its localization by in situ hybridization.

Journal

• Plant and Cell Physiology Supplement

  Plant and Cell Physiology Supplement 2006 (0), 606-606, 2006

  The Japanese Society of Plant Physiologists

Details

• CRID: 1390001205627103744
• NII Article ID: 130006987252
• DOI: 10.14841/jspp.2006.0.606.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed