シアノバクテリアのCa<SUP>2+</SUP>/H<SUP>+</SUP> アンチポ-タ-遺伝子のクロ-ニングとその機能解析

• HOSSAIN Gazi Sakir

  Graduate School of Environmental and Human Sciences, Meijo Univ.

• WADITEE Rungaroon

  Research Institute, Meijo Univ.

• NAKAMURA Tatsunosuke

  Faculty of Pharmacy, Niigata Univ. Pharmacy and Applied Life Science

• SHIKATA Masamitsu

  Shimadzu Co.

• TAKANO Jun

  Shimadzu Co.

• TAKABE Teruhiro

  Research Institute, Meijo Univ.

書誌事項

タイトル別名

• Isolation and Functional Characterization of Ca<SUP>2+</SUP>/H<SUP>+</SUP> antiporters from Cyanobacteria

抄録

Genome sequences of cyanobacteria revealed the presence of a single Ca²⁺/H⁺ antiporter in these organisms. We isolated the putative Ca²⁺/H⁺ antiporter gene from Synechocystis sp. PCC 6803 (synCAX) as well as a homologous gene from a halotolerant cyanobacterium Aphanothece halophytica (apCAX). In contrast to plant vacuolar CAXs, the full length apCAX and synCAX genes complemented the Ca²⁺-sensitive phenotype of an E. coli mutant. ApCAX and synCAX proteins catalyzed specifically the Ca²⁺/H⁺ exchange reaction at alkaline pH. Immunological analysis suggested their localization in plasma membranes. The Synechocystis sp. PCC 6803 cells disrupted of synCAX exhibited lower Ca²⁺ efflux activity and a salt sensitive phenotype. Overexpression of apCAX and synCAX enhanced the salt tolerance of Synechococcus sp. PCC 7942 cells.

収録刊行物

• 日本植物生理学会年会およびシンポジウム　講演要旨集

  日本植物生理学会年会およびシンポジウム　講演要旨集 2004 (0), 115-115, 2004

  日本植物生理学会

詳細情報

• CRID: 1390001205627779456
• NII論文ID: 130006988087
• DOI: 10.14841/jspp.2004.0.115.0
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可