硝酸による非共生型ヘモグロビンの誘導における硝酸還元酵素の関与 Involvement of nitrate reductase activity in nitrate-induced expression of non-symbiotic hemoglobins in cultured rice cells

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Abstract

Non-symbiotic hemoglobins (nsHbs) are ubiquitous in plants. Dicots are known to have two distinct classes of nsHb genes whereas monocots may have one class. Recent microarray work indicated that class 1 nsHb genes are inducible by nitrate in <I>Arabidopsis</I>, though the factors associated with induction of nsHbs by nitrate have not been examined in detail. Expression of two class 1 nsHb genes, <I>ORYsaGLB1a</I> and <I>ORYsaGLB1b</I>, was induced in nitrate treated rice cells in a manner similar to that of the induction of nitrate reductase (NR) activity. When nitrate-treated cells were grown in a medium containing tungstate, an inhibitor for NR activity, the expression of <I>ORYsaGLB1a</I> and <I>ORYsaGLB1b</I> was repressed. Furthermore, mutants defective in NR activity were impaired in the induction of <I>ORYsaGLB1a</I> and <I>ORYsaGLB1b</I> by nitrate. These results suggest that NR activity may be essential for the induction of nsHbs by nitrate in cultured rice cells.

Journal

  • Plant and Cell Physiology Supplement

    Plant and Cell Physiology Supplement 2004(0), 663-663, 2004

    The Japanese Society of Plant Physiologists

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