Absence of the <I>PsbH</I> Gene Product Destabilizes Photosystem II Complex: the Lack of PSII-X Protein in the Thermophilic Cyanobacterium <I>Thermosynechococcus elongatus</I> BP-1.
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- IWAI Masako
- Department of Life Sciences (Biology), Tokyo Univ.
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- KATAYAMA Mitsunori
- Department of Life Sciences (Biology), Tokyo Univ.
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- IKEUCHI Masahiko
- Department of Life Sciences (Biology), Tokyo Univ.
Bibliographic Information
- Other Title
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- 好熱性シアノバクテリア<I>Thermosynechococcus elongatus</I> BP-1のPSII-Hタンパク質の機能解析
Abstract
PSII-H is present in photosystem II (PSII) core complex in cyanobacteria and plants. The role of PSII-H was studied by directed mutagenesis and biochemical analysis in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. The photoautotrophic growth of the psbH-disruptant was much slower than that of wild type. Cells and thylakoids from the mutant showed about half the oxygen-evolving activity of wild type. While the PSII complex of wild type was mainly collected as dimer by anion exchange chromatography, the PSII complex of the psbH-disruptant was not recovered as dimer but as two monomeric forms. One was an inactive PSII complex lacking 33 kDa, cyt c550, and 12 kDa extrinsic proteins. The other was a PSII complex retains the oxygen-evolving activity and the extrinsic proteins. Moreover, PSII-X was absent in the PSII complexes of the psbH-disruptant. These results imply that PSII-H stabilizes the dimeric PSII complex associated with PSII-X and extrinsic proteins.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2004 (0), 213-213, 2004
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205628229376
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- NII Article ID
- 130006988520
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed