Absence of the <I>PsbH</I> Gene Product Destabilizes Photosystem II Complex: the Lack of PSII-X Protein in the Thermophilic Cyanobacterium <I>Thermosynechococcus elongatus</I> BP-1.

DOI

Bibliographic Information

Other Title
  • 好熱性シアノバクテリア<I>Thermosynechococcus elongatus</I> BP-1のPSII-Hタンパク質の機能解析

Abstract

PSII-H is present in photosystem II (PSII) core complex in cyanobacteria and plants. The role of PSII-H was studied by directed mutagenesis and biochemical analysis in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. The photoautotrophic growth of the psbH-disruptant was much slower than that of wild type. Cells and thylakoids from the mutant showed about half the oxygen-evolving activity of wild type. While the PSII complex of wild type was mainly collected as dimer by anion exchange chromatography, the PSII complex of the psbH-disruptant was not recovered as dimer but as two monomeric forms. One was an inactive PSII complex lacking 33 kDa, cyt c550, and 12 kDa extrinsic proteins. The other was a PSII complex retains the oxygen-evolving activity and the extrinsic proteins. Moreover, PSII-X was absent in the PSII complexes of the psbH-disruptant. These results imply that PSII-H stabilizes the dimeric PSII complex associated with PSII-X and extrinsic proteins.

Journal

Details 詳細情報について

  • CRID
    1390001205628229376
  • NII Article ID
    130006988520
  • DOI
    10.14841/jspp.2004.0.213.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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