トリプトファン蓄積イネのメチルジャスモン酸処理による代謝系遺伝子のマイクロアレイ解析

• Dubouzet Joseph

  National Institute of Crop Science<br>Biotechnology Laboratory JST-CREST

• Wakasa Kyo

  National Institute of Crop Science<br>Biotechnology Laboratory JST-CREST

書誌事項

タイトル別名

• Microarray analysis of metabolic gene expression in high tryptophan rice as affected by methyl jasmonate

抄録

Gene expression in Nipponbare and two lines (HW1 and HW5) overexpressing a mutant gene for anthranilate synthase alpha subunit (OASA1D) were evaluated in relation to methyl jasmonate treatment.<br>Two- factorial ANOVA of microarray data revealed that some (1.7% = 371/21495) genes other than OASA1D were differentially expressed in these three genotypes. Genes involved in the biosynthesis of tryptophan from D-arabino-heptulosonate 7-phosphate were upgregulated by exposure to methyl jasmonate but downstream genes that process tryptophan into other metabolites did not show a similarly coordinated response. This may be a reason for the accumulation of tryptophan in OASA1D lines.<br>Genes showing significant differences among genotypes were observed in stress signal transduction pathways and in the biosynthesis of other amino acids, hormones, indoles and flavonoids. Pathway analysis of gene expression in transgenic rice has revealed important insights into the effects of genetic transformation and exposure to methyl jasmonate on the rice transcriptome.

収録刊行物

• 日本植物生理学会年会およびシンポジウム　講演要旨集

  日本植物生理学会年会およびシンポジウム　講演要旨集 2005 (0), 469-469, 2005

  日本植物生理学会

詳細情報

• CRID: 1390001205629032576
• NII論文ID: 130006989410
• DOI: 10.14841/jspp.2005.0.469.0
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可