Overexpression of bacterial catalase in tomato plant chloroplasts enhances oxidative stress tolerance

• EL-Awady Mohamed

  Dept. Appl. Biochem., Fac. Agr., Osaka<br>Pref. Univ.

• Tamoi Masahiro

  Fac. Agr. Kinki Univ.

• Shigeoka Shigeru

  Fac. Agr. Kinki Univ.

• Iwaki Toshio

  Dept. Appl. Biochem., Fac. Agr., Osaka<br>Pref. Univ.

• Wadano Akira

  Dept. Appl. Biochem., Fac. Agr., Osaka<br>Pref. Univ.

抄録

The E.coli gene katE, driven by the promoter of Rubisco small subunit gene of tomato, rbcS3C, was introduced into a tomato (Lycopersicon esculentum Mill.) by Agrobacterium tumefaciens-mediated transformation. Catalase activity in transgenic plant was approx. 3-fold higher than that in wild-type plant. Leaf discs from transgenic plants remained green at 24h after treatment with 1 μM paraquat under moderate light intensity, while leaf discs from wild-type plants showed severe bleaching after the same treatment. In addition, ion leakage from transgenic leaf discs was significantly less than that from wild-type leaf discs at 24h after treatment with 1 μM paraquat and 10 mM H₂O₂, respectively, under moderate light intensity. The protect efficiency of the E.coli catalase in the transgenic plant was checked against different oxidative stresses. The transgenic plants showed relatively higher tolerance for paraquat, exogenous H₂O₂ and cold stress than the wild-type plants but not for salt stress

収録刊行物

• 日本植物生理学会年会およびシンポジウム　講演要旨集

  日本植物生理学会年会およびシンポジウム　講演要旨集 2003 (0), 345-345, 2003

  日本植物生理学会

詳細情報

• CRID: 1390282680606120704
• NII論文ID: 130006989850
• DOI: 10.14841/jspp.2003.0.345.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可