AtMap1タイリングアレイを用いたハイスループット正遺伝学の試み AtMap1: a DNA Microarray for Genomic Deletion Mapping in <i>Arabidopsis thaliana</i>

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We designed a novel tiling array, AtMap1, for genomic deletion mapping. The AtMap1 is a 60-mer oligonucleotide microarray consisting of 42,497 data probes designed from the genomic sequence of <i>Arabidopsis thaliana</i> Col-0. The performance of the AtMap1 array was assessed using the <i>mag2-2</i> mutant, which is a well-studied deletion mutant. Eight of the probes showed 3-fold lower signals in <i>mag2-2</i> than Col-0. Seven of these probes were sequentially located on chromosome 3. This deletion included previously reported deleted region. The other probe was located near the end of chromosome 4. A deletion around the probe was confirmed by PCR. This deletion was newly identified in this study. Thus, we concluded that the AtMap1 array was sufficiently sensitive to identify a deletion without a priori knowledge. Mutation mapping is time-consuming, laborious and costly. The AtMap1 array removes the difficulties.


  • Plant and Cell Physiology Supplement

    Plant and Cell Physiology Supplement 2008(0), 0978-0978, 2008

    The Japanese Society of Plant Physiologists


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