AtMap1: a DNA Microarray for Genomic Deletion Mapping in <i>Arabidopsis thaliana</i>
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- Nagano Atsushi J.
- Graduate School of Science, Kyoto Univ.
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- Fukazawa Mitsue
- National Institute for Basic Biology
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- Nishimura Mikio
- National Institute for Basic Biology
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- Hara-Nishimura Ikuko
- Graduate School of Science, Kyoto Univ.
Bibliographic Information
- Other Title
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- AtMap1タイリングアレイを用いたハイスループット正遺伝学の試み
Abstract
We designed a novel tiling array, AtMap1, for genomic deletion mapping. The AtMap1 is a 60-mer oligonucleotide microarray consisting of 42,497 data probes designed from the genomic sequence of Arabidopsis thaliana Col-0. The performance of the AtMap1 array was assessed using the mag2-2 mutant, which is a well-studied deletion mutant. Eight of the probes showed 3-fold lower signals in mag2-2 than Col-0. Seven of these probes were sequentially located on chromosome 3. This deletion included previously reported deleted region. The other probe was located near the end of chromosome 4. A deletion around the probe was confirmed by PCR. This deletion was newly identified in this study. Thus, we concluded that the AtMap1 array was sufficiently sensitive to identify a deletion without a priori knowledge. Mutation mapping is time-consuming, laborious and costly. The AtMap1 array removes the difficulties.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2008 (0), 0978-0978, 2008
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680607578240
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- NII Article ID
- 130006991796
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed