AtMap1: a DNA Microarray for Genomic Deletion Mapping in <i>Arabidopsis thaliana</i>

DOI

Bibliographic Information

Other Title
  • AtMap1タイリングアレイを用いたハイスループット正遺伝学の試み

Abstract

We designed a novel tiling array, AtMap1, for genomic deletion mapping. The AtMap1 is a 60-mer oligonucleotide microarray consisting of 42,497 data probes designed from the genomic sequence of Arabidopsis thaliana Col-0. The performance of the AtMap1 array was assessed using the mag2-2 mutant, which is a well-studied deletion mutant. Eight of the probes showed 3-fold lower signals in mag2-2 than Col-0. Seven of these probes were sequentially located on chromosome 3. This deletion included previously reported deleted region. The other probe was located near the end of chromosome 4. A deletion around the probe was confirmed by PCR. This deletion was newly identified in this study. Thus, we concluded that the AtMap1 array was sufficiently sensitive to identify a deletion without a priori knowledge. Mutation mapping is time-consuming, laborious and costly. The AtMap1 array removes the difficulties.

Journal

Details 詳細情報について

  • CRID
    1390282680607578240
  • NII Article ID
    130006991796
  • DOI
    10.14841/jspp.2008.0.0978.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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