Characterization of AtMTP4, a zinc transporter in plasma membrane

• Segami Shoji

  Grad. Sch. Bioagr. Sci., Nagoya Univ.

• Kawachi Miki

  Grad. Sch. Bioagr. Sci., Nagoya Univ.

• Kobae Yoshihiro

  Grad. Sch. Bioagr. Sci., Nagoya Univ.

• Iwano Megumi

  Grad. Sch. Biosci., NAIST

• Maeshima Masayoshi

  Grad. Sch. Bioagr. Sci., Nagoya Univ.

Bibliographic Information

Other Title

• 細胞膜に存在する亜鉛輸送体AtMTP4の解析

Abstract

Zinc is a trace element essential for all organisms as a cofactor for various enzymes and proteins. However, excess zinc becomes toxic. We study on zinc transporters in plants. In Arabidopsis thaliana, MTP1 and MTP3 function as Zn²⁺/H⁺ antiporter in vacuolar membrane and play a key role in zinc tolerance. In this study, we investigated MTP4. GFP-tag analysis and subcellular fractionation disclosed that MTP4 is localized in the plasma membrane. Yeast complementation assay using a zinc-hypersensitive strain showed the zinc transport activity of MTP4. Promoter GUS assay revealed that MTP4 is expressed in mature pollens and vascular bundles. Analysis using a construct of own promoter driven MTP4-GFP showed that MTP4 exists in pollen tubes. With consideration of structural similarity to MTP1, MTP4 may function as a zinc efflux transporter in the plasma membrane. We also showed the phenotypic properties of the T-DNA inserted mutant lines.

Journal

• Plant and Cell Physiology Supplement

  Plant and Cell Physiology Supplement 2009 (0), 0827-0827, 2009

  The Japanese Society of Plant Physiologists

Details

• CRID: 1390001205632371840
• NII Article ID: 130006994035
• DOI: 10.14841/jspp.2009.0.0827.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed