CO<SUB>2</SUB> sensing at ocean surface mediated by cAMP in a marine diatom.

• Harada Hisashi

  Marine Biotechnoloy Institute

• Nakajima Kensuke

  Department of Bioscience, Kwansei-Gakuin University

• Sakaue Kunihiro

  Department of Bioscience, Kwansei-Gakuin University

• Kitahara Yuhei

  Department of Bioscience, Kwansei-Gakuin University

• Matsuda Yusuke

  Department of Bioscience, Kwansei-Gakuin University

Bibliographic Information

Other Title

• cAMPが媒介する海洋性珪藻のCO<SUB>2</SUB>センシング機構

Abstract

Chloroplastic carbonic anhydrase in the marine diatom Phaeodactylum tricornutum, PtCA1, is assumed to balance Ci species in the chloroplast. PtCA1 gene (ptca1) is a typical CO₂-responsive gene whose expression is repressed under elevated [CO₂]. In this study, mechanisms of regulation of the ptca1 promoter, Pptca1 was investigated. The core-regulatory region of Pptca1 (downstream -70bp relative to the transcription-start site) comprises of three putative cis-elements (CRE1, P300-binding site, and CRE2). Deletions or substitutions of these elements were carried out, fused to uidA-reporter gene, and introduced into P. tricornutum. Legions in CRE1 and p300bs caused a lack of ptca1 repression even in high CO₂. Moreover, treatment of cells with a cAMP analogue or a cAMP-phosphodiesterase inhibitor efficiently repressed the endogenous Pptca1 even in air, but these repressive effects were disappeared by deleting CRE1 from the Pptca1. The function of cAMP for CO₂ sensing mechanism in marine diatoms will be discussed.

Journal

• Plant and Cell Physiology Supplement

  Plant and Cell Physiology Supplement 2007 (0), 218-218, 2007

  The Japanese Society of Plant Physiologists

Details

• CRID: 1390282680609163904
• NII Article ID: 130006994158
• DOI: 10.14841/jspp.2007.0.218.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed