12-Oxo-phytodienoic acid-Glutathione Conjugate is Transported into the Vacuole in Arabidopsis.
-
- Ohkama-Ohtsu Naoko
- Grad. Agri. Sci., Tokyo Univ. Agri. Tech. Tokyo Univ. Agri. Tech.Women's Future Development Organization
-
- Sasaki-Sekimoto Yuko
- RIKEN Plant Science Center
-
- Oikawa Akira
- RIKEN Plant Science Center
-
- Jikumaru Yusuke
- RIKEN Plant Science Center
-
- Shinoda Shoko
- RIKEN Plant Science Center
-
- Inoue Eri
- RIKEN Plant Science Center
-
- Kamide Yukiko
- RIKEN Plant Science Center
-
- Yokoyama Tadashi
- Grad. Agri. Sci., Tokyo Univ. Agri. Tech.
-
- Hirai Masami Yokota
- RIKEN Plant Science Center
-
- Shirasu Ken
- RIKEN Plant Science Center
-
- Kamiya Yuji
- RIKEN Plant Science Center
-
- Oliver David J.
- Dep. GDCB, Iowa State Univ.
-
- Saito Kazuki
- RIKEN Plant Science Center
Bibliographic Information
- Other Title
-
- シロイヌナズナにおいてジャスモン酸前駆体12-Oxo-phytodienoic acidはグルタチオン抱合体として液胞に輸送される
Abstract
While exogenous toxic compounds such as herbicides are thought to be sequestered into vacuoles in the form of glutathione (GSH) conjugates, little is understood about natural plant products conjugated with GSH. To identify natural products conjugated with GSH in plants, metabolites in the Arabidopsis γ-glutamyl transpeptidase (ggt) 4 knockout mutants that are blocked in the degradation of GSH conjugates in the vacuole were compared with those in wild-type plants. Plants were infected with a pathogen because a phytoalexin in Vigna radiata, was previously shown to conjugate with GSH in vitro (Li et al. 1997) and oxylipins, whose GSH conjugates were detected in vivo, was previously found to be induced by the pathogen (Mueller et al. 2008). Metabolites were analyzed using CE -TOF/MS. Among the metabolites accumulated in ggt4 mutants, one was confirmed to be the 12-oxo-phytodienoic acid (OPDA)-GSH conjugate. OPDA is a precursor of jasmonic acid. OPDA-GSH was increased upon the pathogen infection and this was concomitant with the increase in OPDA. This indicates that a certain portion of OPDA was transported into the vacuole in the form of GSH conjugate.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2011 (0), 0211-0211, 2011
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205632521088
-
- NII Article ID
- 130006994257
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed