Live imaging of chloroplast FtsZ1 filaments, rings, spirals, and motile dot structures in the <I>AtMinE1</I> mutant and overexpressor of <I>Arabidopsis thaliana</I>

• Fujiwara Makoto T.

  RIKEN Dept. of Life Sci., Univ. of Tokyo

• Sekine Kohsuke

  Dept. of Life Sci., Univ. of Tokyo

• Yamamoto Yoshiharu Y.

  RIKEN

• Abe Tomoko

  RIKEN

• Sato Naoki

  Dept. of Life Sci., Univ. of Tokyo

• Itoh Ryuuichi D.

  Fac. of Sci., Univ. of Ryukyus

Bibliographic Information

Other Title

• シロイヌナズナMinE過剰発現体及び変異体における葉緑体FtsZ1-GFPタンパク質のライブ観察

Abstract

Chloroplast division involves the tubulin-related GTPase FtsZ that assembles into a ring structure (Z-ring) at the mid-chloroplast division site, which is where invagination and constriction of the envelope membranes occur. Z-ring assembly is usually confined to the mid-chloroplast site by a well balanced counteraction of the stromal proteins MinD and MinE. The in vivo mechanisms by which FtsZ nucleates at specific sites, polymerises into a protofilament and organises a closed ring of filament bundles remain largely unknown. To clarify the dynamic aspects of FtsZ, we developed a living cell system for simultaneous visualisation of various FtsZ configurations, utilising the Arabidopsis thaliana overexpressor and mutant of the MinE (AtMinE1) gene, which were modified to weakly express green fluorescent protein (GFP) fused to AtFtsZ1-1. Our microscopic data of short filaments, rings, spirals, and motile dot structures of FtsZ1 will be presented.

Journal

• Plant and Cell Physiology Supplement

  Plant and Cell Physiology Supplement 2010 (0), 0362-0362, 2010

  The Japanese Society of Plant Physiologists

Details

• CRID: 1390001205633087616
• NII Article ID: 130006995093
• DOI: 10.14841/jspp.2010.0.0362.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed