Regulation of betaine synthesis by precursor supply and choline monooxygenase expression in <I>Amaranthus tricolor</I>

DOI

Bibliographic Information

Other Title
  • アマランサスのベタイン合成の制御におよぼす前駆体とコリンモノオキシゲナーゼの役割

Abstract

In plants, betaine is synthesized upon abiotic stress via choline oxidation, in which choline monooxygenase (CMO) is a key enzyme. Although it had been thought that betaine synthesis is well regulated to protect plant against abiotic stress, here it is shown that an exogenous supply of precursors such as choline, serine, and glycine in the betaine-accumulating plant Amaranthus tricolor further enhances the accumulation of betaine under salt-stress, but not normal, conditions. Addition of isonicotinic acid hydrazide, an inhibitor of glycine decarboxylase, inhibited the salinity-induced accumulation of betaine. Salt-induced accumulation of Amaranthus tricolor CMO (AmCMO) and betaine were much slower in roots than in leaves. Antisense expression of AmCMO mRNA suppressed the salt-induced accumulation of AmCMO and betaine, but increased the level of choline. The genomic DNA, including the upstream region (1.6 kbp), of AmCMO was isolated.Importance of promoter sequence of CMO and precursor supply were discussed.

Journal

Details 詳細情報について

  • CRID
    1390282680609907712
  • NII Article ID
    130006995252
  • DOI
    10.14841/jspp.2008.0.0470.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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