プロテオミクスによる多層カーボンナノチューブの安全性評価法の可能性

• 羽二生 久夫

  信州大学医学部

• 小山 省三

  信州大学医学部

• 金 隆岩

  信州大学工学部

• 林 卓哉

  信州大学工学部

• 竹内 健司

  信州大学工学部

• 遠藤 守信

  信州大学工学部

書誌事項

タイトル別名

• Potential of Safety Evaluation Method for Malti-Walled Carbon Nanotubes by Proteomic Approach

抄録

We have the alternation of protein expressions using human monoblastic leukemia cells (U937) exposed to multi-walled carbon nanotubes (MWCNTs) to explore the possibility of a new safety evaluation method using the proteomic approach. MWCNTs obtained by the catalytic chemical vapor deposition method were treated with four different temperatures to remove polyaromatic hydrocarbons and iron compounds as catalysts. The cells with MWCNTs were cultured for 4 days, counted, and analyzed by 2-dimensional electrophoresis (2-DE). The protein spots with alteration of expression level were identified by matrix-associated laser desorption/ionization time-of-flight mass spectrometry. To the extent that the processing temperature of the MWCNTs was low, cell proliferation tended to be inhibited. But there were no significant differences in the proliferation of U937 cells which were exposed to MWCNTs treated with the highest temperature. On the other hand, when the MWCNTs were treated with low temperatures, the spots that showed changes in the protein expression levels increased. The intensity of 37 spots on 2-DE gels changed significantly (P < 0.05) on U937 cells exposed to MWCNTs treated with a lower temperature, while MWCNTs treated with the highest temperature changed 20 protein spots. Peptide mass fingerprinting identified 35 of 37 and 16 of 20 protein spots. Five spots were common among all grades of MWCNTs. Some stress-related proteins even changed in the cells exposed to MWCNTs treated with the highest temperature. In regard to safety risk, the proteomic approach could detect biological responses more sensitively than conventional evaluation methods in vitro.

収録刊行物

• 日本プロテオーム学会大会要旨集

  日本プロテオーム学会大会要旨集 2008 (0), 22-22, 2008

  日本プロテオーム学会（日本ヒトプロテオーム機構）

キーワード

• malti-walled carbon nanotube
• safety evaluation
• cell toxicity

詳細情報

• CRID: 1390282680611152000
• NII論文ID: 130006996768
• DOI: 10.14889/jhupo.2008.0.22.0
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可