The effect of norepinephrine on radiation sensitivity in rat ileal epithelial cells

• MATSUU-MATSUYAMA Mutsumi

  Dept. Molec. Pathol., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

• SHICHIJO Kazuko

  Dept. Molec. Pathol., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

• OKAICHI Kumio

  Dept. Radiat. Biophys., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

• NAKAYAMA Toshiyuki

  Dept. Molec. Pathol., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

• NAKASHIMA Masahiro

  Tissue and Histopath. Sec., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

• SEKINE Ichiro

  Dept. Molec. Pathol., Atomic Bomb Dis. Inst., Nagasaki Univ. Grad. Sch. Biomed. Sci.

抄録

We previously reported that the apoptosis index in jejunal crypt cells was significantly greater in spontaneously hypertensive rats than in Wistar-Kyoto rats after X irradiation and that administration of reserpine to induce sympathetic dysfunction of spontaneously hypertensive rats or Wistar-Kyoto rats resulted in a significant suppression of apoptosis. We hypothesized that hyperfunction of the sympathetic nervous system is involved producing the high susceptibility to radiation-induced apoptosis of the jejunal crypt cells. To clarify this hypothesis, we examined the effect of norepinephrine (NE) on cell survival using the clonogenic assay and apoptosis after X-ray irradiation in rat ileal epithelial cells (IEC-18).<BR> NE treatment in rat ileal epithelial cells resulted in the decrease of cell survival in a dose dependent manner (>1µM). A combination of NE 1µM and radiation decreased surviving fraction at 8 Gy from 24.1% to 6.4 % significantly. NE concentration 1µM resulted in increase the apoptotic rate from 3.5 % to 6.0 % 6 h after 8 Gy irradiation (p<0.05). The NE induced cell sensitivity to radiation was attenuated by alpha1-adrenergic antagonist (prazosin) but was not affected by beta-adrenergic antagonist (propranolol).<BR> Our study suggested that NE enhanced sensitivity to radiation in rat epithelial cells and that may be caused through alpha adrenergic receptor.

収録刊行物

• 日本放射線影響学会大会講演要旨集

  日本放射線影響学会大会講演要旨集 2005 (0), 301-301, 2005

  一般社団法人 日本放射線影響学会

詳細情報

• CRID: 1390001205641207552
• NII論文ID: 130007000752
• DOI: 10.11513/jrrsabst.2005.0.301.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可