Histopathological changes in the brain of mouse fetuses by etoposide-administration

• Nam Chunja

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Woo Gye-Hyeong

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Uetsuka Koji

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Nakayama Hiroyuki

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Doi Kunio

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Bibliographic Information

Other Title

• Etoposideに起因されるマウスの胎仔の脳の組織病理学的な変化

Abstract

Etoposide (VP-16), a topoisomerase II inhibitor, is an anti-tumor agent which causes embryotoxicity, exencephaly, encephalocele, anophthalmia and major skeletal malformations of fetuses when administered to dams of rodents. We examined VP-16-induced histopathological changes in the brain of mouse fetuses. Pregnant mice were intraperitoneally treated with VP-16 (4 mg/kg) on 12th day of gestation (GD 12), and fetuses were collected from 1 to 48 hours after treatment (HAT). A moderate decrease of mitotic figures of neuroepithelial cells was observed in the telencephalic wall at 4 HAT. A moderate to marked increase of pyknotic neuroepithelial cells was detected in the brain of VP-16-treated fetuses, and being most prominent from 8 to 24 HAT. These pyknotic cells were also positively stained by TUNEL and cleaved caspase-3 immunostain. It was suggested that excessive neuronal apoptosis was induced in the brain of mouse fetuses treated with VP-16 on GD 12.

Journal

• Annual Meeting of the Japanese Society of Toxicology

  Annual Meeting of the Japanese Society of Toxicology 32 (0), 16-16, 2005

  The Japanese Society of Toxicology

Details

• CRID: 1390001205658580608
• NII Article ID: 130007003554
• DOI: 10.14869/toxp.32.0.16.0
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed