Optical analysis of PKC-dependent modulation at mossy fiber terminals of hippocampus using synaptopHluorin transgenic mice
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- Hikima Takuya
- Dept of Dev Biol and Neurosci, Tohoku Univ Grad Sch of Life Sci, Sendai, Japan
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- Araki Rikita
- Dept of Dev Biol and Neurosci, Tohoku Univ Grad Sch of Life Sci, Sendai, Japan Laboratory for Neuronal Circuit Dynamics, BSI, RIKEN, Wako-shi, Japan
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- Ishizuka Toru
- Dept of Dev Biol and Neurosci, Tohoku Univ Grad Sch of Life Sci, Sendai, Japan
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- Yawo Hiromu
- Dept of Dev Biol and Neurosci, Tohoku Univ Grad Sch of Life Sci, Sendai, Japan
Bibliographic Information
- Other Title
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- SynaptopHluorin トランスジェニックマウスを用いた海馬苔状線維終末におけるPKC依存的開口放出修飾の光学的解析
Abstract
The synaptic transmission at hippocampal mossy fiber (MF) synapses is modulated by protein kinase C (PKC) at the presynaptic terminal. The mechanisms of this modulation were investigated at individual MF terminals. We used a TV-42 transgenic mouse line that expresses synaptopHluorin (SpH), one of optical probes measuring exo-endocytosis, specifically in the hippocampal mossy fiber terminals. The mouse was deeply ether-anesthetized and the brain was removed. The hippocampal slices were made at 400 μm. Individual large mossy fiber terminals were identified under confocal microscopy and the activity-dependent changes of SpH fluorescence were measured. The activity-dependent change of SpH fluorescence was on average increase by phorbol esters, synthetic analogues of diacylglycerol, whereas not by 4α-phorbol, one of inactive analogues. Phorbol esters increased the fusion probability of a vesicle in some boutons (type 1), whereas they preferentially increase the readily releasable pool in others (type 2). Some other synapses are presynaptically silent but become releasable upon exposure to phorbol esters (type 3). These effects were almost completely inhibited by staurosporine, one of PKC antagonists. Thus the PKC-dependent exocytosis modulation is variable from MF synapse to synapse. All animal procedures were conducted in accordance with the guiding principles of Physiological Society of Japan. [J Physiol Sci. 2007;57 Suppl:S154]
Journal
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- Proceedings of Annual Meeting of the Physiological Society of Japan
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Proceedings of Annual Meeting of the Physiological Society of Japan 2007 (0), 154-154, 2007
PHYSIOLOGICAL SOCIETY OF JAPAN
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Keywords
Details 詳細情報について
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- CRID
- 1390282680704004992
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- NII Article ID
- 130007038583
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed