HIV由来レンチウイルスベクターを用いたIn vivoマウス小脳プルキンエ細胞への遺伝子導入

• 寅嶋 崇

  金沢大院・医・生理 金沢大・学際センター・革新脳科学 (独) 科学技術振興機構・さきがけ２１

• 山田 伸明

  金沢大・学際センター・革新脳科学

• 平井 宏和

  金沢大・学際センター・革新脳科学 (独) 科学技術振興機構・さきがけ２１

書誌事項

タイトル別名

• In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors

抄録

Cerebellar Purkinje cells are key elements regulating motor learning and motor coordination. Gene transfer into neurons, followed by the assessment of the effects on neural function, is an effective approach for examining gene function. However, this method has not been used fully in the study of the cerebellum, because of the obstacle of delivering genes into Purkinje cells. To overcome this, we used a human immunodeficiency virus (HIV)-derived lentiviral vector and examined the transduction profile of the vector in the cerebellum. A lentiviral vector expressing GFP was injected into the cerebellar cortex. Seven days after the injection, GFP was predominantly expressed in Purkinje cells. GFP was also expressed, though less efficiently, in other cortical interneurons and Bergmann glias. In contrast to reported findings with other viral vectors, no transduced cells were observed outside of the cerebellar cortex, even in the deep cerebellar nuclei, pontine nuclei and inferior olivary complex, which are synaptically linked with Purkinje cells or granule cells. Thus, when HIV-derived lentiviral vectors were injected into the cerebellar cortex, transduction was limited to the cells in the cerebellar cortex, with the highest tropism for Purkinje cells. These results suggest that HIV-derived lentiviral vectors are useful for the study of gene function in Purkinje cells as well as for application as a gene therapy tool for the treatment of diseases that affect Purkinje cells. [J Physiol Sci. 2006;56 Suppl:S167]

収録刊行物

• 日本生理学会大会発表要旨集

  日本生理学会大会発表要旨集 2006 (0), 167-167, 2006

  一般社団法人　日本生理学会

キーワード

• Cerebellum
• Purkinje cell
• Lentivirus
• HIV vector
• Bergmann glia

詳細情報

• CRID: 1390282680704887936
• NII論文ID: 130007038943
• DOI: 10.14849/psjproc.2006.0.167.1
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可