包括的細胞シミュレータ"simBio"による近位尿細管上皮細胞モデルの構築と水輸送シミュレーション

• 竹内 綾子

  京大・細胞・生体機能シミュレーションプロジェクト

• 原 秀人

  京大・院・薬・動態制御

• 山下 富義

• 橋田 充

  京大・細胞・生体機能シミュレーションプロジェクト 京大・院・薬・動態制御

• 野間 昭典

  京大・細胞・生体機能シミュレーションプロジェクト 京大・院・医・細胞機能制御

書誌事項

タイトル別名

• Simulation of water transport in the proximal tubular cell on the "simBio" platform

抄録

In the proximal tubules, water movement from tubular lumen to peritubular capillary is the passive consequence of active solute reabsorption. This vectorial transport is characterized by a combination of many carrier proteins, expressed differently in polarized membranes, apical and basolateral membranes. In the present study, we successfully reconstructed a mathematical model of proximal tubular epithelial cells, reported by Weinstein (Am J Physiol 263: F784, 1992), on our originally developed cell simulation platform "simBio". When tubular Na⁺ was replaced with impermeable molecule, apical Na⁺ flux into the cell via Na⁺-driven transporter, such as Na⁺-glucose or Na⁺-H₂PO₄⁻ cotransporter and Na⁺/H⁺ exchanger, was greatly reduced. Then intracellular Na⁺ concentration decreased gradually, resulting in the reduction of basolateral Na⁺ efflux via Na⁺-coupled transport processes such as Na⁺/K⁺ pump and Na⁺-3HCO₃⁻ cotransproter. According to the reduction of Na⁺ fluxes in each membrane, water fluxes were also greatly reduced, confirming the significance of osmosis in water reabsorption at the proximal tubules. Furthermore, known observations regarding the change of water transport in response to changes in composition of extracellular fluid, such as reduction of HCO₃⁻ and addition of cyanide and glucose, were well explained by the model. [Jpn J Physiol 55 Suppl:S113 (2005)]

収録刊行物

• 日本生理学会大会発表要旨集

  日本生理学会大会発表要旨集 2005 (0), S113-S113, 2005

  一般社団法人　日本生理学会

キーワード

• proximal tubule
• water transport
• model

詳細情報

• CRID: 1390001205729135104
• NII論文ID: 130007039085
• DOI: 10.14849/psjproc.2005.0.s113.1
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可