カルシウムチャネルブロッカーはヒト好中球のサイトカインによる活性化を阻害する

• 加藤 隆幸

  Dept. Physiol. Med. Osaka City Univ., Osaka, Japan

• 勝部 匡尊

  Dept. Physiol. Med. Osaka City Univ., Osaka, Japan

• 羽藤 文彦

  Dept. Physiol. Med. Osaka City Univ., Osaka, Japan

• 藤田 寿一

  Dept. Physiol. Med. Osaka City Univ., Osaka, Japan

• 北川 誠一

  Dept. Physiol. Med. Osaka City Univ., Osaka, Japan

書誌事項

タイトル別名

• Calcium Channel Blockers Suppress Cytokine-Induced Activation of Human Neutrophils

抄録

Neutrophils play an important role in the progression of atherosclerosis in concert with proinflammatory cytokines. Calcium channel blockers are commonly used for treatment of hypertension, and their pleiotropic effects other than lowering blood pressure are recently recognized. We studied the effects of various calcium channel blockers (amlodipine, nicardipine, cilnidipine, benidipine, efonidipine, nifedipine, azelnidipine, verapamil, and diltiazem) on superoxide (ROS) release, migration, and the signaling pathways in human neutrophils stimulated by GM-CSF or TNF. GM-CSF-induced ROS release was suppressed by amlodipine, nicardipine, and cilnidipine, whereas TNF-induced ROS release was suppressed by amlodipine, nicardipine, cilnidipine, benidipine, efonidipine, nifedipine, and azelnidipine. TNF-induced phosphorylation of ERK and Akt, but not p38 MAPK, was attenuated by nicardipine, cilnidipine, benidipine, efonidipine, and azelnidipine. By contrast, GM-CSF-induced phosphorylation of ERK, p38 MAPK, and Akt was affected by neither blockers. GM-CSF-induced neutrophil migration was also suppressed by amlodipine and nicardipine, but not by azelnidipine, when these blockers were assessed for their effect on neutrophil migration. These findings suggest that some calcium channel blockers can suppress cytokine-induced neutrophil activation, leading to possible prevention of the progression of atherosclerosis, and that activation of the ERK and PI3K/Akt pathways induced by TNF, but not by GM-CSF, is selectively affected by some blockers. [J Physiol Sci. 2008;58 Suppl:S74]

収録刊行物

• 日本生理学会大会発表要旨集

  日本生理学会大会発表要旨集 2008 (0), 074-074, 2008

  一般社団法人　日本生理学会

キーワード

• Calcium channel blocker
• neutrophils
• superoxide
• granulocyte-macrophage colony-stimulating factor
• tumor necrosis factor-α

詳細情報

• CRID: 1390282680706826368
• NII論文ID: 130007039623
• DOI: 10.14849/psjproc.2008.0.074.1
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可