Influence of clove oil and eugenol on muscle contraction of silkworm (<i>Bombyx mori</i>)
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- Kheawfu Kantaporn
- Nanoscience and Nanotechnology Program, The Graduate School, Chiang Mai University
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- Pikulkaew Surachai
- Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University
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- Hamamoto Hiroshi
- Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Teikyo University Institute of Medical Mycology
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- Sekimizu Kazuhisa
- Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo Teikyo University Institute of Medical Mycology
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- Okonogi Siriporn
- Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University Research Center of Pharmaceutical Nanotechnology, Chiang Mai University
抄録
<p>Clove oil is used in fish anesthesia and expected to have a mechanism via glutamic receptor. The present study explores the activities of clove oil and its major compound, eugenol, in comparison with L-glutamic acid on glutamic receptor of silkworm muscle and fish anesthesia. It was found that clove oil and eugenol had similar effects to L-glutamic acid on inhibition of silkworm muscle contraction after treated with D-glutamic acid and kainic acid. Anesthetic activity of the test samples was investigated in goldfish. The results demonstrated that L-glutamic acid at 20 and 40 mM could induce the fish to stage 3 of anesthesia that the fish exhibited total loss of equilibrium and muscle tone, whereas clove oil and eugenol at 60 ppm could induce the fish to stage 4 of anesthesia that the reflex activity of the fish was lost. These results suggest that clove oil and eugenol have similar functional activities and mechanism to L-glutamic acid on muscle contraction and fish anesthesia.</p>
収録刊行物
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 11 (2), 64-69, 2017
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会