Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

• SUMIDA Yuko

  Graduate School of Pharmaceutical Sciences, University of Tokyo

• TSUNODA Makoto

  Graduate School of Pharmaceutical Sciences, University of Tokyo

• FUNATSU Takashi

  Graduate School of Pharmaceutical Sciences, University of Tokyo

Abstract

In this study, the application of a new type of monolithic silica column to the analysis of amino acids in human plasma samples has been investigated. The monolithic silica column (3.0 × 150 mm, MonoTower C18) provided good separation of the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Although the separation ability of this column was not much improved in comparison with the previous monolithic silica columns, it was similar with that of a 3 μm particle packed column. The column with length 150 mm had the same separation property as a tandem connection of columns of lengths 50 mm and 100 mm. After 1000 injections of human plasma samples, the peak widths for the NBD-amino acids were increased by only 8%. Thus, the newly developed monolithic silica column can be potentially applied for faster analysis and/or more efficient separation of biological compounds.

Journal

• CHROMATOGRAPHY

  CHROMATOGRAPHY 38 (3), 135-138, 2017

  The Society for Chromatographic Sciences

Keywords

• <i> </i>Fluorescence
• 4-Fluoro-7-nitro-2,1,3-benzoxadizaole (NBD-F)
• Plasma
• Durability

Details

• CRID: 1390282680721028992
• NII Article ID: 130007040472
• NII Book ID: AA1137755X
• DOI: 10.15583/jpchrom.2017.016
• ISSN: 13483315
• ISSN: 13428284
• NDL BIB ID: 028644702
• Web Site: http://id.ndl.go.jp/bib/028644702
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028644702
• Web Site: https://www.jstage.jst.go.jp/article/jpchrom/38/3/38_2017.016/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed