Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria

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Author(s)

    • FUJIWARA Natsumi
    • Department of Oral Microbiology, Tokushima University Graduate School|Department of Oral Healthcare Promotion, Tokushima University Graduate School
    • MIYAKE Yoichiro
    • Department of Oral Microbiology, Tokushima University Graduate School
    • MURAKAMI Keiji
    • Department of Oral Microbiology, Tokushima University Graduate School
    • NAKAO Michiyasu
    • Department of Molecular Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University
    • TOGUCHI Munehisa
    • Department of Molecular Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University
    • YUMOTO Hiromichi
    • Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School
    • MATSUO Takashi
    • Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School
    • SANO Shigeki
    • Department of Molecular Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University
    • OZAKI Kazumi
    • Department of Oral Healthcare Promotion, Tokushima University Graduate School

Abstract

<b>OBJECTIVE:</b> <i>Lactobacillus reuteri</i> is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by <i>L. reuteri</i> and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria. <br><b>MATERIALS AND METHODS:</b> The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by <i>Fusobacterium nucleatum</i> were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes. <br><b>RESULTS:</b> Among synthesized RRCs, RRC-04,-05,-09,-10,-12,-13, and -14, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-06,-07,-08, and -11, significantly suppressed biofilm formation to 60-80 % of the control. Most RRCs, except for RRC-12 and -13, were not cytotoxic to human oral keratinocytes. <br><b>CONCLUSIONS:</b> The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.

Journal

  • Journal of Oral Health and Biosciences

    Journal of Oral Health and Biosciences 30(1), 8-17, 2017

    Shikoku Society of Dental Research

Codes

  • NII Article ID (NAID)
    130007053971
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    2188-7888
  • Data Source
    IR  J-STAGE 
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