ウコン中のCurcuminの高速液体クロマトグラフィーによる定量 Determination of Curcumin Content of Turmeric by High Performance Liquid Chromatography
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A method of quantitative determination of curcumin in Curcuma longa was established by using high performance liquid chromatography. Curcumin contents of methanol, aqueous methanol-(4 : 6) and water-extracts of turmeric (rhizomes of Curcuma longa L.) were determined by high performance liquid chromatography (HPLC) by use of Nucleosil C<SUB>18</SUB> column as a stationary phase, a mixture of CH<SUB>3</SUB>CN : H<SUB>2</SUB>O : CH<SUB>3</SUB>COOH (51 : 49 : 5) as a mobile phase and benzyl benzoate as an internal standard. The present method is preferable to other analytical methods with regard to accuracy, simplicity and speed.
- YAKUGAKU ZASSHI
YAKUGAKU ZASSHI 101(4), 374-377, 1981
The Pharmaceutical Society of Japan