オルトジアニシジン-ペルオキシダーゼ系によるホモバニリン酸の測定 Determination of Homovanillic Acid Using o-Diamisidine-Peroxidase System

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Abstract

A new and sensitive method for the fluorometric determination of homovanillic acid using o-dianisidine-peroxidase system was established. Under the optimum conditions, the calibration curve was linear from 0.5μg/ml to 20μg/ml (0.5-20μg/tube) of homovanillic acid, and coefficients of variation was below 5 percents. Salicylic acid, glucose, urea, p-hydroxyphenylacetic acid, and homogentisic acid did not interfer with the value of homovanillic acid, but the coexistence of uric acid, dopamine, and serotonine caused clearly decreases in the homovanillic acid value by the present method.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 104(5), 540-543, 1984

    The Pharmaceutical Society of Japan

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