ENHANCED EXPRESSION OF A 37, 000-DALTON PROTEIN IN HUMAN LUNG CANCERS

• OHKUBO Michiko

  Divisions of Biochemistry, Chiba Cancer Center Research Institute and Hospital

• SAWADA Kinya

  Divisions of Thoracic Disease, Chiba Cancer Center Research Institute and Hospital

• SAKIYAMA Shigeru

  Divisions of Biochemistry, Chiba Cancer Center Research Institute and Hospital

抄録

When proteins in the tissue extracts of lung cancers were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE), a 37, 000-dalton protein (37K) was detected as a prominent form in all cases thus far examined. This protein was barely detectable or its amount was very small in the normal lung tissue extracts. Two-dimensional PAGE of lung tumor extracts showed 37K as a major spot with an isoelectric point of 7.6. An antibody against 37K was raised in a rabbit by injecting purified 37K. Immunoblot analysis of normal lung and lung tumor tissues revealed that the affinity-purified anti-37K antibody specifically recognized 37K and that the intensities of immunostaining corresponded to those of Coomassie brilliant blue staining.

収録刊行物

• Ｊａｐａｎｅｓｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｃａｎｃｅｒ　Ｒｅｓｅａｒｃｈ　ＧＡＮＮ

  Ｊａｐａｎｅｓｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｃａｎｃｅｒ　Ｒｅｓｅａｒｃｈ　ＧＡＮＮ 77 (6), 546-553, 1986

  日本癌学会

キーワード

• Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
• Tissue extracts
• Lung cancer

詳細情報

• CRID: 1390001206305456128
• NII論文ID: 130007310022
• DOI: 10.20772/cancersci1985.77.6_546
• ISSN: 09105050
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可