New Complement Fixation Test with Peroxidase-Labeled Complement Clq for Direct and Quantitative Determination of Antibodies to Herpes Simplex Virus
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- TAGUCHI Fumiaki
- Department of Microbiology, School of Hygienic Sciences, Kitasato University
抄録
An enzyme-labeled complement fixation (ELISA-CF) test for the direct and quantitative determination of complement fixing (CF) antibodies has been developed. This paper described the introduction of the ELISA-CF test that used peroxidase-labeled Clq component of complement to detect CF antibodies which had reacted with herpes simplex virus (HSV), as a virus model. Equal volumes of heat-inactivated serum and the peroxidase-labeled Clq (P*-Clq) were simultaneously added to wells of microplates which had been coated with HSV CF antigen or with cell control antigen. The enzymatic activities of P*-Clq bound to the immune complex were determined photometrically. The ELISA-CF test allows processing of serum specimens in a 3-hr operation, with procedural simplicity and increased specificity and sensitivity compared with the conventional CF test.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 32 (11), 1167-1173, 1988
財団法人 学会誌刊行センター
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詳細情報 詳細情報について
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- CRID
- 1573668928035469312
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- NII論文ID
- 130007313123
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles