Establishment of DNA methylation patterns of the <i>Fibrillin1</i> (<i>FBN1</i>) gene in porcine embryos and tissues

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Author(s)

    • ARAI Yoshikazu
    • Laboratory of Genomic Function Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan|Laboratory of Veterinary Biochemistry and Molecular Biology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
    • UMEYAMA Kazuhiro
    • Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan|Meiji University International Institute for Bio-Resource Research (MUIIBR), Kanagawa 214-8571, Japan
    • TAKEUCHI Kenta
    • Laboratory of Genomic Function Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
    • OKAZAKI Natsumi
    • Laboratory of Genomic Function Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
    • HICHIWA Naomi
    • Laboratory of Genomic Function Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
    • YASHIMA Sayaka
    • Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
    • NAKANO Kazuaki
    • Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
    • NAGASHIMA Hiroshi
    • Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan|Meiji University International Institute for Bio-Resource Research (MUIIBR), Kanagawa 214-8571, Japan
    • OHGANE Jun
    • Laboratory of Genomic Function Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan

Abstract

<p> DNA methylation in transcriptional regulatory regions is crucial for gene expression. The DNA methylation status of the edges of CpG islands, called CpG island shore, is involved in tissue/cell-type-specific gene expression. Haploinsufficiency diseases are caused by inheritance of one mutated null allele and are classified as autosomal dominant. However, in the same pedigree, phenotypic variances are observed despite the inheritance of the identical mutated null allele, including <i>Fibrillin1</i> (<i>FBN1)</i>, which is responsible for development of the haploinsufficient Marfan disease. In this study, we examined the relationship between gene expression and DNA methylation patterns of the <i>FBN1</i> CpG island shore focusing on transcriptionally active hypomethylated alleles (Hypo-alleles). No difference in the DNA methylation level of <i>FBN1</i> CpG island shore was observed in porcine fetal fibroblast (PFF) and the liver, whereas <i>FBN1</i> expression was higher in PFF than in the liver. However, Hypo-allele ratio of the <i>FBN1</i> CpG island shore in PFF was higher than that in the liver, indicating that Hypo-allele ratio of the <i>FBN1</i> CpG island shore likely correlated with <i>FBN1</i> expression level. In addition, oocyte-derived DNA hypermethylation in preimplantation embryos was erased until the blastocyst stage, and re-methylation of the <i>FBN1</i> CpG island shore was observed with prolonged <i>in vitro</i> culture of blastocysts. These results suggest that the establishment of the DNA methylation pattern within the <i>FBN1</i> CpG island shore occurs after the blastocyst stage, likely during organogenesis. In conclusion, Hypo-allele ratios of the <i>FBN1</i> CpG island shore correlated with <i>FBN1</i> expression levels in porcine tissues.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 63(2), 157-165, 2017

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130007314629
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • Data Source
    J-STAGE 
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