スピロヘータ遺伝子を標的としたPCRによるアコヤガイ赤変病の疫学的調査 An Epidemiological Study of Akoya Oyster Disease Using Polymerase Chain Reaction Targeting Spirochaetes Genes

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Author(s)

Abstract

<p>In a previous study, a member of Spirochaeta was presumed to cause Akoya oyster disease (AOD), and <i>Candidatus</i> Maribrachyspira akoyae was proposed as the putative causative agent. With the aim of performing an epidemiological study of AOD, polymerase chain reaction (PCR) assays were developed using the primers designed from shotgun metagenomic sequences identified as having high homology to Spirochaetes genes by a BLAST search. ​PCR assays with six primer sets produced specific target amplicons (<i>n </i>= 63–67) in pearl oysters collected from five AOD-endemic areas (<i>n</i> = 88), but did not in pearl oysters (<i>n</i> = 36) collected from three AOD-free areas. ​All individuals (<i>n</i> = 17) with the color index a-value above 3.0, which is an index of AOD development, were positive for all 6 primer sets in PCR. There was a positive correlation between the a-value and the results of PCR. ​These results show that the previous identification of Spirochaeta as an etiological agent of AOD is reasonable. ​Sequencing of amplification products confirmed only one nucleotide substitution in the PCR products, suggesting that the pathogenic causative agent of AOD has limited genetic diversity.</p>

Journal

  • Fish Pathology

    Fish Pathology 53(2), 63-70, 2018

    The Japanese Society of Fish Pathology

Codes

  • NII Article ID (NAID)
    130007386159
  • NII NACSIS-CAT ID (NCID)
    AN00063165
  • Text Lang
    ENG
  • ISSN
    0388-788X
  • NDL Article ID
    029152270
  • NDL Call No.
    Z18-994
  • Data Source
    NDL  J-STAGE 
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