Long-term Culture of a Single Neuron using a Microwell with a Porous SiN Membrane

DOI Web Site 5 References
  • Zenmyo Yusuke
    Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology
  • Morisako Isamu
    Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology
  • Yasuda Takashi
    Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology

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Other Title
  • SiN多孔膜を有するマイクロウェルを用いた単一ニューロンの長期培養

Abstract

<p>For long-term culture of a single neuron, many astrocytes and a single neuron were co-cultured on both sides of a 1 µm thick silicon nitride membrane which has many microholes of 3 µm in diameter. After astrocytes were cultured on the back side of the membrane for 1 week, a neuron was seeded on the front side of the membrane of a microwell bottom and cultured for 5 weeks. By fluorescently staining VGLUT1 (vesicular glutamate transporter 1) which is localized at a presynaptic terminal, we found that the number of synapses formed by a single neuron drastically increases within the first 2 weeks and keeps high values for weeks afterward.</p>

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