Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments

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  • Morono Yuki
    Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC
  • Hoshino Tatsuhiko
    Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC
  • Terada Takeshi
    Marine Works Japan Ltd.
  • Suzuki Taketo
    Koken Ltd.
  • Sato Takahiro
    Koken Ltd.
  • Yuasa Hisashi
    Koken Ltd.
  • Kubota Yuji
    Koken Ltd.
  • Inagaki Fumio
    Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Geobiotechnology Group, Research and Development Center for Submarine Resources, JAMSTEC Research and Development Center for Ocean Drilling Science, JAMSTEC

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<p>Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.</p>

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