<b>A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal </b><b>cavity </b>
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- TSUZUKI Satoshi
- Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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- KIMOTO Yusaku
- Laboratory of Nutrition Chemistry Department of Food Science and Biotechnology, Faculty of Agriculture, Kyoto University
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- LEE Shinhye
- Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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- SUGAWARA Tatsuya
- Laboratory of Technology of Marine Bioproducts, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- MANABE Yuki
- Laboratory of Technology of Marine Bioproducts, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- INOUE Kazuo
- Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity
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Abstract
<p>Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.</p>
Journal
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- Biomedical Research
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Biomedical Research 39 (3), 117-129, 2018-06-01
Biomedical Research Press