DNA Photocrosslinking Using 3-Vinylcarbazole Derivatives in Two-color Detection of Methylcytosine

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Author(s)

    • Nakamura Shigetaka
    • Department of Advanced Science and Technology, Japan Advanced Institute of Science and Technology
    • Nakajima Ryo
    • Department of Advanced Science and Technology, Japan Advanced Institute of Science and Technology
    • Fujimoto Kenzo
    • Department of Advanced Science and Technology, Japan Advanced Institute of Science and Technology

Abstract

<p>The methylated DNA base 5-methylcytosine (<sup>m</sup>C) is the most important factor in the epigenetic regulation of gene expression. 3-cyanovinylcarbazole nucleoside (<sup>CNV</sup>K) photocrosslinks with <sup>m</sup>C more rapidly than with cytosine (C). In the present study, we evaluated the effect of the substituents of various 3-vinylcarbazole derivatives on <sup>m</sup>C- and C-mediated DNA photocrosslinking for the quantitative detection of <sup>m</sup>C. We also demonstrated the quantitative detection of <sup>m</sup>C via photocrosslinking using 3-vinylcarbazole derivatives on a DNA chip. The results suggest that this photocrosslinking reaction has potential for the selective detection of <sup>m</sup>C in DNA strands via photocrosslinking using 3-cyanovinylcarbazole derivatives.</p>

Journal

  • Chemistry Letters

    Chemistry Letters 47(7), 875-877, 2018

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130007402740
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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