Development of microsatellite markers for the endangered orchid <i>Calanthe izu-insularis</i> (Orchidaceae)

• Nakahama Naoyuki

  Graduate School of Arts and Sciences, The University of Tokyo Graduate School of Agriculture, Kyoto University

• Ito Azusa

  Graduate School of Agriculture, Kyoto University

• Kaneko Shingo

  Graduate School of Agriculture, Kyoto University Division of Environment System Management, Fukushima University

• Matsuki Yu

  Kawatabi Field Science Center, Graduate School of Agricultural Science, Tohoku University

• Suyama Yoshihisa

  Kawatabi Field Science Center, Graduate School of Agricultural Science, Tohoku University

• Hayano Azusa

  Graduate School for Health Care Science, Kyoto Prefectural University of Medicine Wildlife Research Center of Kyoto University

• Murayama Miho

  Wildlife Research Center of Kyoto University

• Isagi Yuji

  Graduate School of Agriculture, Kyoto University

Bibliographic Information

Other Title

• Development of microsatellite markers for the endangered orchid Calanthe izu-insularis (Orchidaceae)
• Development of microsatellite markers for endangered orchid Calanthe izu-insularis (Orchidaceae)

Abstract

Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1–6 (mean, 2.35) and 0.00–0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 93 (1), 31-35, 2018-02-01

  The Genetics Society of Japan

Keywords

• <i>Calanthe</i>
• conservation
• endangered orchid
• natural hybridization
• next-generation sequencing

Details

• CRID: 1390845712974000768
• NII Article ID: 130007404291
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.17-00021
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 029217015
• PubMed: 29343671
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029217015
• Web Site: https://www.jstage.jst.go.jp/article/ggs/93/1/93_17-00021/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed