A Safe, Simple, and Facile Staining Method Using Polysiloxanes for High-Contrast Visualization of Gelator Aggregates by Transmission Electron Microscopy
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- Selected Paper : A Safe, Simple, and Facile Staining Method Using Polysiloxanes for High-Contrast Visualization of Gelator Aggregates by Transmission Electron Microscopy
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Abstract
The staining of TEM samples using Si atoms was investigated using aggregates of loose gels formed by twelve structurally different gelators in several solvents. Thirteen commercially available siloxanes were used as stains. TEM images of non-stained and OsO4-stained samples of molecular aggregates formed by the dodecamethylenediamide of N-methacryloyl-oxyethylaminocarbonyl-L-isoleucine in 1-propanol were poorly defined and low-contrast. However, the image of a methacryloyloxypropyl-terminated polydimethylsiloxane (S1)-stained sample was characterized by very clear bundles of fine fibers. The staining effect was explained by the wrapping of fibers, the stabilizing of the individual fibers, and reinforcing by S1. An S1 concentration of more than 5 mgmL(-1) was found to be necessary for satisfactory contrast. S1 was successfully applied to the observation of aggregates of eleven other gelators. S1 worked universally as an aggregate stain regardless of the gelator or solvent polarity. The staining effect was observed for other siloxanes. This effect was found to depend on the molecular weight of the siloxane (>1,000) rather than the kind of siloxane employed. Energy-dispersive X-ray spectroscopy indicated that the molecules of S1 gather on the surface of the fibers during drying, wrapping them. The results indicate that the present staining method guarantees reproducibility and universality.
Article
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN.91(7):1176-1185(2018)
Journal
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- BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
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BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 91 (7), 1176-1185, 2018-07
CHEMICAL SOC JAPAN
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Details 詳細情報について
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- CRID
- 1050846638692585856
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- NII Article ID
- 130007404786
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- NII Book ID
- AA00580132
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- ISSN
- 00092673
- 13480634
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- HANDLE
- 10091/00021970
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- NDL BIB ID
- 029089286
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- NDL
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- CiNii Articles