Comparison of gene expression levels of <i>appA</i>, <i>ppsR</i>, and <i>EL368</i> in <i>Erythrobacter litoralis</i> spheroplasts under aerobic and anaerobic conditions, and under blue light, red light, and dark conditions
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- Nishino Koki
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
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- Takahashi Sawako
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
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- Nishida Hiromi
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
抄録
<p>We compared the gene expression levels of the blue-light-responsive genes, appA (encoding photosynthesis promoting protein AppA), ppsR (encoding photosynthesis suppressing protein PpsR), and EL368 (encoding a blue-light-activated histidine kinase with a light, oxygen, or voltage domain) between aerobic and anaerobic conditions in spheroplasts of the aerobic photosynthetic bacterium Erythrobacter litoralis. The spheroplasts conducted photosynthesis under red light but not under blue light. All three blue-light-responsive genes showed higher expression under aerobic conditions than under anaerobic conditions under blue light. In contrast, under red light, although the expression level of appA was higher in the presence of oxygen than in the absence of oxygen, the expression levels of ppsR and EL368 were similar in the presence and absence of oxygen. Our findings demonstrate that the expression of blue-light-responsive genes is strongly affected by oxygen in E. litoralis spheroplasts.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 64 (3), 117-120, 2018
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
