Comparative Mapping of the <i>ASTRINGENCY</i> Locus Controlling Fruit Astringency in Hexaploid Persimmon (<i>Diospyros kaki</i> Thunb.) with the Diploid <i>D. lotus</i> Reference Genome

• Nishiyama Soichiro

  Graduate School of Agriculture, Kyoto University

• Onoue Noriyuki

  Institute of Fruit Tree and Tea Science, NARO

• Kono Atsushi

  Institute of Fruit Tree and Tea Science, NARO

• Sato Akihiko

  Institute of Fruit Tree and Tea Science, NARO

• Ushijima Koichiro

  Graduate School of Environmental and Life Science, Okayama University

• Yamane Hisayo

  Graduate School of Agriculture, Kyoto University

• Tao Ryutaro

  Graduate School of Agriculture, Kyoto University

• Yonemori Keizo

  Graduate School of Agriculture, Kyoto University

Bibliographic Information

Other Title

• Comparative Mapping of the ASTRINGENCY Locus Controlling Fruit Astringency in Hexaploid Persimmon (Diospyros kaki Thunb.) with the Diploid D. lotus Reference Genome

Abstract

Persimmon (Diospyros kaki) is a tree crop species that originated in East Asia, consists mainly of hexaploid individuals (2n = 6x = 90) with some nonaploid individuals. One of the unique characteristics of persimmon is the continuous accumulation of proanthocyanidins (PAs) in its fruit until the middle of fruit development, resulting in a strong astringent taste even at commercial fruit maturity. Among persimmon cultivars, pollination-constant and non-astringent (PCNA) types cease PA accumulation in early fruit development and become non-astringent at commercial maturity. PCNA is an allelic trait to non-PCNA and is controlled by a single locus called the ASTRINGENCY (AST) locus. Previous segregation analyses indicated that the AST locus shows hexasomic inheritance; a recessive allele, ast, at this locus confers PCNA. Here, we report a shuttle mapping approach to delimit the AST locus region in the hexaploid persimmon genome by using D. lotus, a diploid relative of D. kaki, as a reference. A D. lotus F₁ population of 333 individuals and 296 D. kaki siblings segregating for the PCNA trait were used to map the AST region using haplotype-specific markers covering the AST region. This indicated that the AST locus is syntenic to an approximately 915-kb region of the D. lotus genome. In this 915-kb region, we found several candidates for AST that were revealed from the fruit transcriptome of a population segregating for the PCNA trait. These results could provide important clues for the isolation of AST in hexaploid persimmon.

Journal

• The Horticulture Journal

  The Horticulture Journal 87 (3), 315-323, 2018

  The Japanese Society for Horticultural Science

Keywords

• candidate gene
• chromosome walking
• polyploidy
• proanthocyanidins

Details

• CRID: 1390282763025750272
• NII Article ID: 130007420593
• NII Book ID: AA12708073
• DOI: 10.2503/hortj.okd-140
• ISSN: 21890110
• ISSN: 21890102
• NDL BIB ID: 029097414
• Web Site: http://id.ndl.go.jp/bib/029097414
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029097414
• Web Site: https://www.jstage.jst.go.jp/article/hortj/87/3/87_OKD-140/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed