Lipid Nanodisc Formation using Pxt-5 Peptide Isolated from Amphibian (Xenopus tropicalis) Skin, and its Altered Form, Modify-Pxt-5
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- Ikeda Yuri
- Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST) Faculty of Science and Technology, Tokyo University of Science
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- Taira Toshiaki
- Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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- Sakai Kenichi
- Faculty of Science and Technology, Tokyo University of Science
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- Sakai Hideki
- Faculty of Science and Technology, Tokyo University of Science
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- Shigeri Yasushi
- Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Imura Tomohiro
- Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
書誌事項
- タイトル別名
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- Lipid Nanodisc Formation using Pxt-5 Peptide Isolated from Amphibian (<i>Xenopus tropicalis</i>) Skin, and its Altered Form, Modify-Pxt-5
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抄録
<p>Nanodiscs are self-assembled discoidal nanoparticles composed of amphiphilic α-helical scaffold proteins or peptides that accumulate around the circumference of a lipid bilayer. In this study, Pxt-5, which is an antimicrobial peptide isolated from the skin of Xenopus tropicalis, and its modified peptide (Modify-Pxt-5) were synthesized by solid-phase peptide synthesis (SPPS).Their surface properties, which are an important factor in inducing nanodisc formation, were investigated by circular dichroism (CD) spectroscopy, surface tension measurement, phospholipid vesicle clearance assay, and negative-staining transmission electron microscopy (NS-TEM). The α-helicity of Pxt-5 (8.4%) improved drastically to 45.6% by four amino-acid substitutions (Modify-Pxt-5). Both the peptides, having hydrophobic and hydrophilic faces, behaved like general surfactants, and the surface activity of Modify-Pxt-5 (CAC: 9.5×10–5 M, γCAC: 30.3 mN·m–1) was much higher than that of Pxt-5 (CAC: 7.9×10–5 M, γCAC: 38.1 mN·m–1). A turbid L-α-dimyristoylphosphatidylcholine (DMPC) vesicle solution (T = 0.3%) quickly turned transparent upon addition of Pxt-5 or Modify-Pxt-5. After twelve hours, the transmittance of vesicle solution with Modify-Pxt-5 (T = 96.2%) was found to be higher than that of vesicle solution with Pxt-5 (T = 83.5%), and then the micro-solubilized solutions were observed by NS-TEM. Interestingly, nanodisc structures were found in the vicinity of DMPC vesicles in both the images, and the average diameter of the nanodiscs was 11.2 ± 6.0 nm for those containing Pxt-5 and 10.8 ± 5.8 nm for those containing Modify-Pxt-5. It was also found that Modify-Pxt-5 effectively self-assembles into nanodiscs compared to Pxt-5 without any substitutions.</p>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 67 (8), 1035-1041, 2018
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282763026216064
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- NII論文ID
- 130007429988
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 029116549
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- PubMed
- 30012902
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可