Nematode Muscles Project in Spaceflight Experiment

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<p>Our previous spaceflight experiment CERISE showed that gene and protein expression levels of muscular components, cytoskeleton, and mitochondrial enzymes are altered in space flown wild-type <i>C. elegans</i>. To confirm and clarify whether the <i>C. elegans</i> muscle fibers and mitochondrial network are physically altered in response to microgravity, this Nematode Muscles project was designed with wild-type and several mutant lines with GFP expression. This investigation also studied whether microgravity could affect the insulin/IGF-1 (Insulin-like growth factor -1) and/or TGF-β signaling by imaging DAF-16::GFP fusion protein. Wild-type and several mutants were grown in a culture bag kept under microgravity or 1<i>G</i> centrifuge conditions on board ISS for 4 days starting from L1 larva. All samples were fixed on board and recovered, to be analyzed on the earth. The worms did not grow well in the μ<i>G</i> culture bag probably due to unexpected air bubbles. Therefore, DAF-16 activation observed in larval worms in μ<i>G</i> and not in 1<i>G</i> may be attributed to starvation instead of μ<i>G</i> response. In 1<i>G</i> samples, we could successfully find normal mitochondrial network. We also found that chemical fixation using CFA is an effective method for preservation of GFP containing <i>C. elegans</i> in space environment.</p>


  • Biological Sciences in Space

    Biological Sciences in Space 32(0), 6-10, 2018

    Japanese Society for Biological Sciences in Space


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