Probiotic potential of <i>Lactobacillus</i> isolates of chicken origin with anti-<i>Campylobacter</i> activity

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Author(s)

    • DEC Marta
    • Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
    • NOWACZEK Anna
    • Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
    • URBAN-CHMIEL Renata
    • Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
    • STĘPIEŃ-PYŚNIAK Dagmara
    • Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
    • WERNICKI Andrzej
    • Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland

Abstract

<p>Campylobacteriosis is currently the most frequent zoonosis in humans and the main source of infection is contaminated poultry meat. As chickens are a natural host for <i>Campylobacter</i> species, one strategy to prevent infection in humans is to eliminate these bacteria on poultry farms. A study was conducted to evaluate the probiotic potential of 46 <i>Lactobacillus</i> isolates from chickens faeces or cloacae. All lactobacilli were able to produce active compounds on solid media with antagonistic properties against <i>C. jejuni</i> and <i>C. coli</i>, with <i>L. salivarius</i> and <i>L. reuteri</i> exhibiting particularly strong antagonism. The cell-free culture supernatants had a much weaker inhibitory effect on the growth of <i>Campylobacter</i>, and the neutralization of organic acids caused them to completely lose their inhibitory properties. The ability to produce H<sub>2</sub>O<sub>2</sub> was exhibited by 93% of isolates; most of isolates had a hydrophobic surface, showed excellent survival at pH 2.0 or 1.5, and displayed tolerance to bile; 50% isolates displayed the ability to biofilm formation. Determination of MICs of various antibiotics showed that as much as 80.4% of <i>Lactobacillus</i> isolates were resistant to at least one antimicrobial agent. Seven ultimately selected isolates that met all the basic criteria for probiotics may have potential application in reducing <i>Camylobacter</i> spp. in chickens and thus prevent infections in both birds and humans.</p>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 80(8), 1195-1203, 2018

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130007431858
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • Data Source
    J-STAGE 
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