Effect of ε-Poly-L-lysine on a Glucose Sensor Based on Glucose Oxidase and Ferricyanide Ion

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Abstract

<p>ε-Poly-L-lysine (εPL) is a homopolymer of L-lysine residues with linkages between the α-carboxyl and ε-amino groups. εPL exists as a polycationic species under acidic and neutral conditions, and can form a polyion complex with negatively charged glucose oxidase (GOx). We previously reported that εPL significantly promoted a GOx enzymatic reaction using ferricyanide ion ([Fe(CN)<sub>6</sub>]<sup>3−</sup>) as the oxidant. Here, we construct a GOx-immobilized electrode using εPL and glutaraldehyde, and show that [Fe(CN)<sub>6</sub>]<sup>3−</sup> can efficiently act as the mediator in this electrode. Ferricyanide ion failed to function adequately as the mediator when bovine serum albumin or other polyamines (<i>e.g.</i>, polyallylamine, α-poly-L-lysine) were used instead of εPL. The GOx-immobilized electrode using εPL successfully responded to glucose even when the [Fe(CN)<sub>6</sub>]<sup>3−</sup> concentration was as low as 1 mM, and exhibited a wide dynamic range of up to several tens of mM. Thus, εPL is considered to be a useful additive for glucose sensors based on the [Fe(CN)<sub>6</sub>]<sup>4−/3−</sup>-mediated GOx catalytic current.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 34(8), 947-951, 2018

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130007432042
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    029154028
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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