イネの酢酸処理に伴うGABA生合成とその蓄積 Biosynthesis and accumulation of GABA in rice plants treated with acetic acid

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Abstract

<p>イネ(<i>Oryza sativa</i>)は干ばつで枯死するとその後に給水しても復活することはないが,酢酸水溶液を与えたイネは,乾燥ストレスで一度地上部が枯れながらも,再給水すると新たに地上部を伸長させる特異な乾燥耐性を獲得する.処理中の酢酸代謝を解明するために,<sup>13</sup>Cで標識した酢酸をイネに処理し,LC-MS及び<sup>13</sup>C-NMRで<sup>13</sup>C標識された酢酸代謝物を追跡した.根抽出物のLC-MS及び<sup>13</sup>C-NMRの分析結果から,処理された酢酸はイネ体内に取り込まれ,グルタミン酸デカルボキシラーゼ(GAD)によってγ-アミノ酪酸(GABA)に代謝されると示唆された.酢酸処理したイネのGABA蓄積量を調べると,根に続いて地上部でGABAが蓄積され,また地上部のGABA蓄積量に比例して乾燥ストレスに対する生存率が増加した.以上から,地上部のGABA蓄積が酢酸によるイネの乾燥耐性に寄与する可能性がある.</p>

<p>Rice seedlings (<i>Oryza sativa</i>) that have died from drought cannot be rescued by watering afterward, but pre-treatment with exogenous acetic acid enabled the plants to produce shoots again after being watered (hereinafter referred to as "drought resilience"). To elucidate the metabolism of acetic acid, we treated rice plants with <sup>13</sup>C-labeled acetic acid and traced <sup>13</sup>C-labeled metabolites using LC-MS and <sup>13</sup>C-NMR techniques. The LC-MS and <sup>13</sup>C-NMR spectral data of the root extracts indicated that the acetic acid treatment was absorbed into the plants and then was metabolized to gamma-aminobutyric acid (GABA) by glutamic acid decarboxylase (GAD). GABA accumulation in the roots took place in advance of that in the shoots, and the survival rate against drought stress increased in proportion to the amount of GABA accumulated in the shoots. Therefore, GABA accumulation in shoots may be a key step in drought resilience induced by the acetic acid treatment.</p>

Journal

  • Journal of Pesticide Science

    Journal of Pesticide Science 43(3), 214-219, 2018

    Pesticide Science Society of Japan

Codes

  • NII Article ID (NAID)
    130007437401
  • NII NACSIS-CAT ID (NCID)
    AA11818622
  • Text Lang
    ENG
  • ISSN
    1348-589X
  • NDL Article ID
    029353591
  • NDL Call No.
    Z78-A770
  • Data Source
    NDL  J-STAGE 
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