Interleukin-6 Expression in Three-dimensional Tumor Microenvironment using HSC-4 Cells

• NAKANISHI Tamaki

  Graduate School of Dentistry (Oral and Maxillofacial Surgery),

• TSUJI Kaname

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• MATSUSHIMA Yuki

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• YAMADA Koji

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• MATSUMOTO Kazuhiro

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• YOSHIDA Hiroaki

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• ISEKI Tomio

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

• SHIMIZU Hideo

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan, Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan

• MORITA Shosuke

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

抄録

Reproduction of tumor microenvironment in vivo using the conventional 2- or 3-dimensional culture method is difficult because of the lack of extracellular matrix (ECM) and intercellular interaction. In this study, we tried to establish a 3-dimensional tumor environment using spheroids of oral cancer-derived cells. The Interleukin-6 (IL-6) expression level was high in the spheroids on measurement using the real-time RT-PCR method showing a difference from that in single cells. A representative inflammatory cytokine, IL-6, increases the activity of many types of cancer-derived cells, being involved in tumor formation and metastasis, and similar findings were noted in HSC-4 cells derived from human squamous cell carcinoma of the tongue, for which analysis of phenomena in the cellular interface in 3-dimensionally formed masses is expected. It is highly possible that this method is applicable for studies on the carcinogenicity of nanomaterials represented by asbestos.

収録刊行物

• Nano Biomedicine

  Nano Biomedicine 10 (1), 21-25, 2018

  ナノ・バイオメディカル学会

キーワード

• spheroid
• IL-6
• HSC-4 cells

詳細情報

• CRID: 1390282763035048064
• NII論文ID: 130007438756
• DOI: 10.11344/nano.10.21
• ISSN: 21854734
• ISSN: 18835198
• Web Site: https://search.jamas.or.jp/link/ui/2019103301
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可