EVALUATION OF IL-17 EXPRESSION IN CHRONIC ITP USING IMMUNOHISTOCHEMICAL ANALYSIS

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  • OKAMOTO Naoko
    Department of Pathology, Showa University School of Medicine
  • HOMMA Mayumi
    Department of Pathology, Showa University School of Medicine
  • NAGUMO Tasuku
    Department of Pathology, Showa University School of Medicine
  • KAWAGUCHI Yukiko
    Department of Pathology, Showa University School of Medicine
  • KABASAWA Nobuyuki
    Department of Pathology, Showa University School of Medicine
  • TAZAWA Sakiko
    Department of Pathology, Showa University School of Medicine
  • SHIOZAWA Eisuke
    Department of Pathology, Showa University School of Medicine
  • YAMOCHI Toshiko
    Department of Pathology, Showa University School of Medicine
  • TATE Genshu
    Department of Pathology, Showa University School of Medicine
  • TAKIMOTO Masafumi
    Department of Pathology, Showa University School of Medicine
  • UTO Yui
    Division of Hematology, Department of Medicine, Showa University School of Medicine
  • HATTORI Norimichi
    Division of Hematology, Department of Medicine, Showa University School of Medicine
  • NAKAMAKI Tsuyoshi
    Division of Hematology, Department of Medicine, Showa University School of Medicine
  • YAMAMOTO Shohei
    Department of Pediatrics, Showa University Fujigaoka Hospital
  • ISOYAMA Keiichi
    Department of Pediatrics, Showa University Fujigaoka Hospital

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Other Title
  • 免疫性血小板減少性紫斑病（ITP）症例の骨髄組織におけるIL-17発現に関する免疫組織化学的検討
  • メンエキセイ ケッショウバン ゲンショウセイ シハンビョウ(ITP)ショウレイ ノ コツズイ ソシキ ニ オケル IL-17 ハツゲン ニ カンスル メンエキ ソシキ カガクテキ ケントウ

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Abstract

Immune thrombocytopenic purpura （ITP） is characterized by inhibition/destruction of megakaryocytes in bone marrow （BM） and/or platelets in the spleen and liver. Interleukin-（IL-）17-mediated cells contribute to the imbalance of cellular immunity in the pathogenesis of ITP. Therefore, we examined samples of BM clots to observe the morphological change of megakaryocytes and to test whether IL-17-mediated immunological changes occur in the BM of patients with ITP.　We enrolled 33 patients who were diagnosed with chronic ITP. BM clots were obtained before treatment and stained with hematoxylin and eosin stains for morphological examination and the following markers: CD3, CD4, CD8, CD20, CD25, CD68, CD163, and IL-17. Pathological findings were compared between the ITP patient samples and 11 control samples from patients with malignant lymphoma without infiltration of lymphoma cells. Univariate analysis revealed that patients with ITP had increased numbers of cells expressing CD68, CD163, and IL-17 compared with the control subjects （P ＜0.05）. There were significant correlations between CD68 and CD163 expression levels, and these markers also showed correlations with IL-17 expression．IL-17 expression showed the same distribution as these cells’ expression in immunohistochemical （IHC） staining. Our results indicate that macrophages and monocytes may be involved in deregulated expression of IL-17 in BM of ITP. We suggest that BM examination and IHC staining in patients with ITP may lead to a better understanding of the mechanism of ITP and the pathological diagnosis.

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