TRPV4 Channel-Regulated ATP Release Contributes to γ-Irradiation-Induced Production of IL-6 and IL-8 in Epidermal Keratinocytes

• Ohsaki Airi

  Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Tanuma Sei-ichi

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Tsukimoto Mitsutoshi

  Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science

抄録

External stimuli, such as radiation, induce inflammatory cytokine and chemokine production in skin, but the mechanisms involved are not completely understood. We previously showed that the P2Y11 nucleotide receptor, p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) all participate in interleukin (IL)-6 production induced by γ-irradiation. Here, we focused on the transient receptor potential vanilloid 4 (TRPV4) channel, which is expressed in skin keratinocytes and has been reported to play a role in inflammation. We found that irradiation of human epidermal keratinocytes HaCaT cells with 5 Gy of γ-rays (¹³⁷Cs: 0.75 Gy/min) induced IL-6 and IL-8 production. HaCaT cells treated with TRPV4 channel agonist GSK1016790A also showed increased IL-6 and IL-8 production. In both cases, IL-6/IL-8 production was not increased at 24 h after stimulation, but was increased at 48 h. ATP was released from cells exposed to γ-irradiation or TRPV4 channel agonist, and the release was suppressed by TRPV4 channel inhibitors. The γ-irradiation-induced increase in IL-6 and IL-8 production was suppressed by apyrase (ecto-nucleotidase), NF157 (selective P2Y11 receptor antagonist) and SB203580 (p38 MAPK inhibitor). GSK1016790A-induced inhibitor of kappa B-alpha (IκBα) decomposition, which causes NF-κB activation was suppressed by NF157 and SB203580, and γ-irradiation-induced IκBα decomposition was suppressed by TRPV4 channel inhibitors. Our results suggest that γ-irradiation of keratinocytes induces ATP release via activation of the TRPV4 channel, and then ATP activates P2Y11 receptor and p38 MAPK-NF-κB signaling, resulting in IL-6/IL-8 production.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 41 (10), 1620-1626, 2018-10-01

  公益社団法人 日本薬学会

キーワード

• γ-ray
• transient receptor potential vanilloid 4 channel
• interleukin (IL)-6
• IL-8
• ATP
• P2Y11 receptor

詳細情報

• CRID: 1390845713003740032
• NII論文ID: 130007492935
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b18-00361
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 029238845
• PubMed: 30022772
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029238845
• Web Site: https://www.jstage.jst.go.jp/article/bpb/41/10/41_b18-00361/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019149480
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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