Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery

• Kodama Yukinobu

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Noda Ryo

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Sato Kayoko

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Harasawa Hitomi

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Kurosaki Tomoaki

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Nakagawa Hiroo

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Nakamura Tadahiro

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Kitahara Takashi

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Muro Takahiro

  Department of Hospital Pharmacy, Nagasaki University Hospital

• Sasaki Hitoshi

  Department of Hospital Pharmacy, Nagasaki University Hospital

抄録

Folate receptors are overexpressed on the surface cancer cells. We successfully constructed a new gene delivery vector of methotrexate (MTX)-coated plasmid DNA–polyethylenimine (pDNA–PEI) complexes (PEI complexes) by electrostatic binding. The stable anionic nanoparticle was optimized at MTX charge ratios of 120 or more. pDNA–PEI–MTX complexes (MTX complexes) demonstrated gene expression efficiency as high as cationic pDNA–PEI complexes in the mouse melanoma cell line, B16-F10. The MTX complexes were taken up by the cell-specific uptake mechanisms via the folate receptor. MTX-coated complexes are useful as endocytosis ligands. The MTX120 complexes exhibited no blood aggregation. The transgene efficiency of MTX120 complexes in the liver and spleen after their intravenous administration was higher than that of PEI complexes. Therefore, MTX complexes are expected as a new gene vector in the future.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 41 (10), 1537-1542, 2018-10-01

  公益社団法人 日本薬学会

キーワード

• methotrexate
• gene delivery
• ternary complex
• DNA

詳細情報

• CRID: 1390845713002935552
• NII論文ID: 130007494219
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b18-00144
• ISSN: 13475215
• ISSN: 09186158
• HANDLE: 10069/38631
• NDL書誌ID: 029238526
• PubMed: 30270323
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/1368
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029238526
• Web Site: https://www.jstage.jst.go.jp/article/bpb/41/10/41_b18-00144/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019149467
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可